DAO 49:199-206 (2002)  -  doi:10.3354/dao049199

Complete sequence and structure of ribosomal RNA gene of Heterosporis anguillarum

Shu-Jen Tsai1, Guang-Hsiung Kou2, Chu-Fang Lo2, Chung-Hsiung Wang1,*

1Department of Entomology, National Taiwan University, Taipei 106, Taiwan
2Department of Zoology, National Taiwan University, Taipei 106, Taiwan
*Corresponding author. E-mail:

ABSTRACT: The ribosomal RNA (rRNA) gene region of the microsporidium Heterosporis anguillarum has been examined. Complete DNA sequence data (4060 bp, GenBank Accession No. AF402839) of the rRNA gene of H. anguillarum are presented for the small subunit gene (SSU rRNA: 1359 bp), the internal transcribed spacer (ITS: 37 bp), and the large subunit gene (LSU rRNA: 2664 bp). The secondary structures of the H. anguillarum SSU and LSU rRNA genes are constructed and described. This is the first complete sequence of an rRNA gene published for a fish-infecting microsporidian species. In the phylogenetic analysis, the sequences, including partial SSU rRNA, ITS, and partial LSU rRNA sequences of the fish-infecting microsporidia, were aligned and analysed. The taxonomic position of H. anguillarum as suggested by Lom et al. (2000; Dis Aquat Org 43:225-231) is confirmed in this paper.

KEY WORDS: Heterosporis anguillarum · Microsporidium · rRNA · Secondary structure

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