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Diseases of Aquatic Organisms

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DAO 51:77-80 (2002)  -  doi:10.3354/dao051077

Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

Zerong You1,*, E. Cesar B. Nadala Jr.2, Jinsheng Yang1, Marielle C. W. van Hulten3, Philip C. Loh1

1Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA
2Department of Haematology, University of Cambridge, EABC Site, Long Road, Cambridge CB2 2PT, United Kingdom
3Laboratory of Virology, Wageningen Agricultural University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

ABSTRACT: A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.

KEY WORDS: White spot syndrome virus · 27.5 kDa structural protein · Rabbit polyclonal antiserum

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