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Diseases of Aquatic Organisms

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DAO 53:203-210 (2003)  -  doi:10.3354/dao053203

Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups

D. M. Stone1,*, W. Ahne2, K. L. Denham1, P. F. Dixon1, C. T.-Y. Liu1, A. M. Sheppard1, G. R. Taylor1, K. Way1

1The Centre for the Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom
2Institute of Zoology, Fishery Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Munich, Kaulbachstrasse 37, 80539 Munich, Germany

ABSTRACT: RT-PCR methods have been applied to the detection and sequencing of the glycoprotein gene of putative spring viraemia of carp viruses (SVCV) and pike fry rhabdoviruses (PFRV), including isolates from tench, grass carp, roach, bream and false harlequin, sheatfish and orfe. Phylogenetic analysis of a 550 nucleotide (nt) region of the glycoprotein gene identified 4 groups, I to IV. Significantly, the majority of viruses previously identified as PFRV formed a distinct cluster (Genogroup IV) which shared <80% nucleotide identity with the PFRV reference strain (Genogroup III). The similarity between another PFRV-like virus isolated from grass carp and representatives of Genogroups III and IV was also <80%, indicating that this virus belonged to a third group (Genogroup II). All of the putative SVC viruses were assigned to a 4th group (Genogroup I), sharing <61% nucleotide identity with viruses in Genogroups II to IV.

KEY WORDS: Rhabdovirus · PFRV · SVCV · Sequence analysis · Genogroups

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