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Diseases of Aquatic Organisms

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DAO 55:11-16 (2003)  -  doi:10.3354/dao055011

Molecular characterisation of Neoparamoeba strains isolated from gills of Scophthalmus maximus

Ivan Fiala1,2, Iva Dyková1,2,*

1Institute of Parasitology, Academy of Sciences of the Czech Republic and
2Faculty of Biological Sciences, University of South Bohemia, Brani$ovská 31, 370 05 >eské Budejovice, Czech Republic
*Corresponding author. Email:

ABSTRACT: Small subunit ribosomal RNA gene sequences were determined for 5 amoeba strains of the genus Neoparamoeba Page, 1987 that were isolated from gills of Scophthalmus maximus (Linnaeus, 1758). Phylogenetic analyses revealed that 2 of 5 morphologically indistinguishable strains clustered with 6 strains identified previously as N. pemaquidensis (Page, 1970). Three strains branched as a clade separated from N. pemaquidenis and N. aestuarina (Page, 1970) clades. Our analyses suggest that these 3 strains could be representatives of an independent species. In a more comprehensive eukaryotic tree, strains belonging to Neoparamoeba spp. formed a monophyletic group with a sister-group relationship to Vannella anglica Page, 1980. They did not cluster with Gymnamoebae of the families Hartmannellidae, Flabellulidae, Leptomyxidae or Amoebidae presently available in GenBank.

KEY WORDS: Paramoeba · Neoparamoeba · SSU rDNA · Phylogenetic position

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