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Diseases of Aquatic Organisms

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DAO 55:253-258 (2003)  -  doi:10.3354/dao055253

Propagation of infectious yellow head virus particles prior to cytopathic effect in primary lymphoid cell cultures of Penaeus monodon

Wanchai Assavalapsakul, Duncan R. Smith*, Sakol Panyim

Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Salaya, Nakorn Pathom 73170, Thailand
*Corresponding author. Email:

ABSTRACT: Yellow head virus is a highly pathogenic agent that can cause a fatal disease in several species of penaeid shrimps. Using a primary cell culture and an in vitro quantal assay (TCID50), this study sought to determine the propagation profile of yellow head virus after inoculation at a low multiplicity of infection in the lymphoid tissue (oka organ) of Penaeus monodon. Detectable levels of virus were present as early as 24 h post-inoculation. Maximal viral yields were reached by 4 d post-infection, approximately 24 h after the onset of a detectable cytopathic effect, which was normally observable at 3 d post-inoculation. The methodology provides a useful tool for studying yellow head virus-host-cell interactions.

KEY WORDS: Penaeus monodon · Yellow head virus · YHV · Cell culture · Oka organ

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