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Diseases of Aquatic Organisms

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DAO 59:69-73 (2004)  -  doi:10.3354/dao059069

White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments

Dong-Chun Yan1, Shuang-Lin Dong1,*, Jie Huang2, Xiao-Ming Yu1, Min-Yi Feng1, Xiang-Yi Liu1

1Mariculture Research Laboratory, Fisheries College, Ocean University of China, Qingdao 266003, PR China
2Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China
*Corresponding author. Email:

ABSTRACT: White spot syndrome virus (WSSV) was detected by PCR-dot blot hybridization in rotifer resting eggs from shrimp Penaeus chinensis culture-pond sediments. It was also detected in rotifers hatched from those eggs. Surface disinfection before analysis indicated that WSSV was probably present within the resting eggs. Results suggested that rotifer resting eggs may be an overwintering reservoir for WSSV in shrimp ponds.

KEY WORDS: Rotifer resting eggs · White spot syndrome virus · Transmission route

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