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Diseases of Aquatic Organisms

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DAO 60:11-20 (2004)  -  doi:10.3354/dao060011

Protection of Atlantic salmon Salmo salar against infectious pancreatic necrosis after DNA vaccination

Aase B. Mikalsen1, Jacob Torgersen2, Peter Aleström2, Anne-Lill Hellemann3, Erling-Olaf Koppang3, Espen Rimstad1,*

1Department of Food Safety and Infection Biology, and
2Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, PO Box 8146 Dep, 0033 Oslo, Norway
3GenoMar asa, Oslo Research Park, Gaustadalléen 21, 0349 Oslo, Norway
*Corresponding author. Email:

ABSTRACT: Although vaccines against infectious pancreatic necrosis (IPN) based on inactivated virus or recombinant structural viral proteins are commercially available, the protection is not complete and the disease is still a problem for the Atlantic salmon Salmo salar farming industry. In the present study, 5 different plasmids that expressed whole or parts of the large open reading frames (ORF) of Segment A of the IPN virus (IPNV) were constructed. The plasmids were shown to express proteins in cell cultures and in zebrafish Danio rerio in vivo. The specificities of the expressed proteins were confirmed by staining with IPNV-specific monoclonal antibodies (MAb) The plasmids were then used alone or in different combinations to vaccinate groups of Atlantic salmon, which subsequently were challenged in an experimental assay for IPN. A high level of protection was induced only by the plasmid combination that contained a plasmid expressing all the large ORF polyprotein.

KEY WORDS: DNA-vaccination · Salmo salar · Infectious pancreatic necrosis

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