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Diseases of Aquatic Organisms

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DAO 60:253-257 (2004)  -  doi:10.3354/dao060253

Safety and protective effect of a disinfectant (STEL water) for white spot syndrome viral infection in shrimp

Jong-Hwan Park1, Seung-Hyeok Seok1, Sun-a Cho1, Min-Won Baek1, Hui-young Lee1, Dong-Jae Kim1, Han-Yun Kim2, Se-Ok Chang3, Jae-Hak Park1,*

1Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology,Seoul National University, Seoul 151-742, Korea
2Choonwae Humantech Corporation, 34, Heohyeon-dong 2 ga, Choong-gu, Seoul, 100-876, Korea
3Shin Chon Feed co., Ltd., 660-18, Majeon-dong, Seo-gu, Incheon 404-260, Korea
*Corresponding author. Email:

ABSTRACT: The efficacy of STEL water for protection against white spot syndrome virus (WSSV) infection was evaluated using shrimp. The LC50 of residual chlorine (Cl) in STEL water for broodstock and 2-mo-old shrimp were 2.3 and 3.2 ppm, respectively. All 2-month-old shrimp raised in seawater containing more than 40 µl 2l–1 of a WSSV-infected tissue homogenate died within 3 d post-exposure (dpe). Thus, a 10-fold dose of 400 µl 2l–1 was used in the disinfection tests. Low concentrations of STEL water effectively prevented mortality of shrimp at this challenge dose. All 2-month-old shrimp exposed to seawater with 400 µl of viral homogenate disinfected with STEL water at Cl concentrations over 0.125 ppm for 1 and 10 min, lived until 5 dpe. With 5-mo-old shrimp, all positive control shrimps died within 3 dpe, whereas most shrimp reared in seawater disinfected with STEL water for 1 h before addition of homogenate lived until 5 dpe. Results suggested that continuous disinfection of seawater with STEL water may be effective for preventing WSSV infection in shrimp.

KEY WORDS: STEL water · WSSV · shrimp · disinfection

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