Inter-Research > DAO > v62 > n3 > p191-196  
Diseases of Aquatic Organisms

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DAO 62:191-196 (2004)  -  doi:10.3354/dao062191

Experimental transmission and tissue tropism of Macrobrachium rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV)

A. S. Sahul Hameed1,*, K. Yoganandhan1, J. Sri Widada2, J. R. Bonami2

1Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore District, Tamil Nadu, India
2Pathogens and Immunity, CNRS/UM II, Universite Montpellier II, Case 080, Place Eugene Bataillon, 34095 Montpellier Cedex 5, France

ABSTRACT: White tail disease (WTD) was found to be a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. The causative organisms have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Experimentally transmitted to healthy animals, they caused 100% mortality in post-larvae but failed to cause mortality in adult prawns. The RT-PCR assay revealed the presence of both viruses in moribund post-larvae and in gill tissue, head muscle, stomach, intestine, heart, hemolymph, pleopods, ovaries and tail muscle, but not in eyestalks or the hepatopancreas of experimentally infected adult prawns. The presence of these viruses in ovarian tissue indicates the possibility of vertical transmission. Pleopods have been found to be a suitable organ for detecting these viruses in brooders using the RT-PCR technique.

KEY WORDS: Macrobrachium rosenbergii · Macrobrachium rosenbergii nodavirus · RT-PCR · Pathogenicity · Tissue tropism

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