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Diseases of Aquatic Organisms

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DAO 62:239-244 (2004)  -  doi:10.3354/dao062239

Pseudocohnilembus persalinus (Ciliophora: Scuticociitida) is an additional species causing scuticociliatosis in olive flounder Paralichthys olivaceus

Sung Mi Kim1, Jae Bum Cho1, Eun Hye Lee1, Se Ryun Kwon1, Sung Koo Kim2, Yoon Kwon Nam3, Ki Hong Kim1,*

1Department of Aquatic Life Medicine, 2Faculty of Food Science and Biotechnology, and 3Department of Aquaculture, Pukyong National University, Pusan 608-737, Korea
*Corresponding author. Email:

ABSTRACT: In the present study, Pseudocohnilembus persalinus was first reported as a species causing scuticociliatosis in olive flounder Paralichthys olivaceus. Based on the stained specimens, P. persalinus was clearly differentiated from Uronema marinum, which has been shown to be a cause of scuticociliatosis in farmed olive flounder in Korea from its characteristic oral infraciliature structure. The 1754 bp small subunit ribosomal RNA (SS rRNA) gene sequence of P. persalinus showed 95% homology with the partial sequence of P. hargisi SS rRNA. Moreover, multiplex PCR based on the species-specific amplification of the SS rRNA gene sequence enabled us to distinguish P. persalinus from U. marinum in a simple and rapid manner. P. persalinus was clearly differentiated from U. marinum even when the host was infected simultaneously with both species. These data suggest that the multiplex PCR procedure would make it possible to avoid the cumbersome and time-consuming procedures of morphological analysis for the definitive identification of ciliates.

KEY WORDS: Pseudocohnilembus persalinus · Scuticociliatosis · Olive flounder · Morphology · SS rRNA gene · Multiplex PCR

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