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Diseases of Aquatic Organisms

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DAO 64:159-162 (2005)  -  doi:10.3354/dao064159

Complete small subunit rRNA gene sequence of the scuticociliate Miamiensis avidus pathogenic to olive flounder Paralichthys olivaceus

Sung-Ju Jung*, Shin-Ichi Kitamura, Jun-Young Song, Il-Yong Joung, Myung-Joo Oh

Department of Aqualife Medicine, Yosu National University, San 96-1, Dunduck, Yosu, Chunnam 550-749, Korea

ABSTRACT: Eight isolates of Miamiensis avidus (scuticociliates) were collected from olive flounder Paralichthys olivaceus with symptoms of severe ulcers and haemorrhages at several culture farms in 1999 and 2003. Cloned strains were produced and the complete small subunit ribosomal RNA gene (SSU rRNA) of each strain was sequenced for classification and phylogenic study. The SSU rRNA is 1759 bp in length and the sequence was deposited in the GenBank under accession number AY550080. All 8 strains exhibited the same sequence, but this sequence did not match any previously deposited scuticociliate SSU rRNA sequence. Phylogenetic analysis placed Miamiensis avidus in a sister lineage to Cohnilembus verminus, Pseudocohnilembus hargisi and P. marinus.

KEY WORDS: Scuticociliatida · Miamiensis avidus · Small subunit rRNA · Olive flounder ·Paralichthys olivaceus · Phylogeny

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