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Diseases of Aquatic Organisms

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DAO 66:81-85 (2005)  -  doi:10.3354/dao066081

Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes

Yan Liang1,2,3, Jie Huang1,2,*, Xiao-Ling Song1, Pei-Jun Zhang2, Huai-Su Xu4

1Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,PR China
2Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory for Sustainable Utilizationof Marine Fisheries Resources, Ministry of Agriculture, Qingdao 266071, PR China
3Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China
4Ocean University of China, Qingdao 266003, PR China
*-Corresponding author. Email: address: Yellow Sea Fisheries Research Institute

ABSTRACT: White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)-labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.

KEY WORDS: Viral attachment protein · White spot syndrome virus · WSSV · Shrimp · VP28

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