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Diseases of Aquatic Organisms

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DAO 66:153-157 (2005)  -  doi:10.3354/dao066153

Viremia during infectious salmon anemia virus infection of Atlantic salmon is associated with replicating virus in leucocytes

Emeka Moneke, Basil O. Ikede, Frederick S. B. Kibenge*

Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edwards Island C1A 4P3, Canada
*Corresponding author. Email:

ABSTRACT: In situ hybridization (ISH) was used to investigate the presence of viral mRNA in different fractions of blood cells of Atlantic salmon between 6 and 12 d following experimental infection with infectious salmon anemia virus (ISAV). Using a riboprobe targeting ISAV segment 7 mRNA, hybridization signals were observed only in leucocytes in buffy coat smears from samples collected from 8 to 12 d post-infection (dpi). None of the red blood cell smears from any sample showed ISH signal. These observations allow us to conclude that viremia in ISAV infection is associated with virus replication in leucocytes, and that erythrocytes are not target cells for virus replication.

KEY WORDS: ISAV · Cell-associated viremia · In situ hybridization · Atlantic salmon

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