Inter-Research > DAO > v66 > n3 > p265-270  
Diseases of Aquatic Organisms

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DAO 66:265-270 (2005)  -  doi:10.3354/dao066265

Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus

I. S. Bright Singh1,*, M. Manjusha1, S. Somnath Pai1, Rosamma Philip2

1Centre for Fish Disease Diagnosis and Management, and 2Department of Marine Biology, Microbiology and Biochemistry,Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, Kochi – 682 016, Kerala, India

ABSTRACT: Fenneropenaeus indicus could be protected from white spot disease (WSD) caused by white spot syndrome virus (WSSV) using a formalin-inactivated viral preparation (IVP) derived from WSSV-infected shrimp tissue. The lowest test quantity of lyophilized IVP coated onto feed at 0.025 g–1 (dry weight) and administered at a rate of 0.035 g feed g–1 body weight d–1 for 7 consecutive days was sufficient to provide protection from WSD for a short period (10 d after cessation of IVP administration). Shrimp that survived challenges on the 5th and 10th days after cessation of IVP administration survived repeated challenges although they were sometimes positive for the presence of WSSV by a polymerase chain reaction (PCR) assay specific for WSSV. These results suggest that F. indicus can be protected from WSD by simple oral administration of IVP.

KEY WORDS: Penaeus indicus · Penaeid shrimp · White spot syndrome virus · Vaccination

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