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Diseases of Aquatic Organisms

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DAO 67:183-190 (2005)  -  doi:10.3354/dao067183

Comparison of two birnavirus–rhabdovirus coinfections in fish cell lines

S. Rodriguez1, M. Alonso2, S. I. Pérez-Prieto1,*

1Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas (CSIC), C/Ramiro de Maeztu 9, 28040, Madrid, Spain
2Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis 97330, Oregon, USA
*Corresponding author. Email:

ABSTRACT: Aquabirnaviruses, such as the infectious pancreatic necrosis virus (IPNV), Novirhabdoviruses, such as the infectious hematopoiteic necrosis virus (IHNV) and the viral hemorrhagic septicemia virus (VHSV), cause considerable losses to the salmonid industry worldwide. Coinfections of 2 viruses have been described, but the interactions between rhabdoviruses and birnaviruses have not been examined closely. Using virus titration, flow cytometry and RT-PCR assays, we compared the effect of IPNV on the replication of IHNV and VHSV in tissue culture cells. RT-PCR assays indicated that simultaneous infection of IPNV with VHSV does not affect the replication of the rhabdovirus either in the first or successive passages; the infective titers were similar in single and double infections. In contrast, coinfection of IPNV with IHNV induced a fall in infectivity, with reduced expression of IHNV viral antigens in BF-2 cells from Lepomis macrochirus and a loss of 4.5 log10 units of the infective titer after 3 successive passages. It was possible to stimulate BF-2 cells to produce significant interferon-like activity against IHNV but not against VHSV.

KEY WORDS: Infectious pancreatic necrosis virus · Infectious hematopoiteic necrosis virus · Viral hemorrhagic septicemia virus · Coinfections · Fish viruses

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