Diseases of Aquatic Organisms
DAO is a hybrid research journal on all aspects of disease phenomena in aquatic organisms.
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DOI: https://doi.org/10.3354/dao069249
copiedPolyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression
- Parin Chaivisuthangkura
- Thanawan Tejangkura
- Sombat Rukpratanporn
- Siwaporn Longyant
- Weerawan Sithigorngul
- Paisarn Sithigorngul
ABSTRACT: Capsid protein genes VP1 and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification.
KEYWORDS
Parin Chaivisuthangkura (Co-author)
- Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Thanawan Tejangkura (Co-author)
- Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Sombat Rukpratanporn (Co-author)
- Center of Excellence for Marine Biotechnology at Chulalongkorn University, National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok 10330, Thailand
Siwaporn Longyant (Co-author)
- Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Weerawan Sithigorngul (Co-author)
- Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Paisarn Sithigorngul (Corresponding Author)
- Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand