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Diseases of Aquatic Organisms

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DAO 69:175-183 (2006)  -  doi:10.3354/dao069175

Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD

José M. Rodríguez1, Sonia López-Romalde1,2, Roxana Beaz1, M. Carmen Alonso2, Dolores Castro2, Jesús L. Romalde1,*

1Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, Campus Sur s/n., 15782 Santiago de Compostela, Spain
2Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos, 29071 Málaga, Spain
*Corresponding author. Email:

ABSTRACT: Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clams and causes heavy economic losses. In this study, 28 V. tapetis strains isolated from 5 different hosts were intraspecifically characterized by 3 different polymerase chain reaction- (PCR-) based typing methods: enterobacteria repetitive intergenic consensus (ERIC)-PCR, repetitive extragenic palindromic (REP)-PCR and randomly amplified polymorphic DNA (RAPD)-PCR. Cluster analysis of genetic profiles obtained from these molecular techniques clearly showed the existence of 3 genetic groups strongly correlated to the host origin. The first group was formed by 23 V. tapetis strains isolated from Manila clam Ruditapes philippinarum, 1isolated from venus clam Venerupis aurea, and 1 isolated from common cockle Cerastoderma edule, all collected from France and Spain. The second group was formed by 2 strains isolated from carpet-shell clam R. decussatus cultured in the northwest of Spain. The third group was composed of 1strain isolated from Atlantic halibut Hippoglossus hippoglossus from the UK. We concluded that the 3 typing methods based on PCR were useful for the intraspecific typing of V. tapetis strains, and that they can potentially be used as a fast and reliable tool for epidemiological studies in the future.

KEY WORDS: Vibrio tapetis · RAPD · ERIC-PCR · REP-PCR · Typing

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