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Diseases of Aquatic Organisms

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DAO 69:255-258 (2006)  -  doi:10.3354/dao069255

White tail disease of the giant freshwater prawn Macrobrachium rosenbergii in Thailand

Kalidoss Yoganandhan1,2,*, Manee Leartvibhas3, Supamas Sriwongpuk4, Chalor Limsuwan4

1Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
2National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
3Shrimp Culture Research Center, Charoen Pokphand Foods Public Company, 82/2 Rama 2 Rd., Samut Sakhon 74000, Thailand
4Department of Fisheries Biology, Faculty of Fisheries, Kasetsart University, Pahonyothin Rd., Bangkok 19000, Thailand

ABSTRACT: White tail disease (WTD) of the freshwater prawn Macrobrachium rosenbergii has recently been the cause of high mortalities in Thai prawn farms. The causative agents of this disease in other countries are M. rosenbergii nodavirus (MrNV) and extra small virus (XSV), which are usually detected using reverse transcriptase-polymerase chain reaction (RT-PCR) protocols. Using RT-PCR, most Thai post-larvae (PL) samples showing gross signs of WTD tested positive for MrNV but only a few were positive for XSV. In contrast, all tested brooder samples were positive for both MrNV and XSV. The possibility that brooders infected with MrNV and XSV could transmit the viruses to larvae and PL should be examined. Cloning, sequencing and comparison of deduced amino acid sequences of RT-PCR amplicons of WTD samples from Thailand with those of MrNV and XSV previously reported from the French West Indies and China revealed that the MrNV were closely related but not identical while those from XSV were identical. This is the first report of MrNV and XSV from Thailand.

KEY WORDS: White tail disease · Macrobrachium rosenbergii nodavirus · Extra small virus ·RT-PCR detection · Brooder

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