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Diseases of Aquatic Organisms

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DAO 70:71-79 (2006)  -  doi:10.3354/dao070071

Partial two-dimensional gel electrophoresis (2-DE) maps of Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408

Gee-Wook Shin1, K. J. Palaksha1, Hyang-Hee Yang1, Yong-Seung Shin1, Young-Rim Kim1, Eun-Young Lee1, Myung-Joo Oh2, Tae-Sung Jung1,*

1Laboratory of Fish & Shellfish Diseases, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, ROK
2Department of Fish Pathology, Yosu National University, Yosu 550-747, ROK
*Corresponding author. Email:

ABSTRACT: To examine the proteomes of 2 important causative agents of fish streptococcosis, Streptococcus iniae ATCC29178 and Lactococcus garvieae KG9408, we used 2-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to generate 2-DE maps of these type strains. Silver-stained 2-DE gels of S. iniae ATCC29178 and L. garvieae KG9408 revealed ~320 and 300 spots, respectively, and immobilized pH gradient strips (13 cm, pH 4 to 7) revealed that the majority of the detected spots were concentrated in the pH range of 4.5 to 5.5. The spots were randomly selected from the 2-DE profiles and identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry. The majority of the identified proteins were functionally related to energy and carbohydrate metabolism (e.g. enolase ATPase, glyceraldehyde-3-phosphate dehydrogenase) or translation and translocation (e.g. elongation factor G, elongation factor Tu, DNA-directed RNA polymerase alpha chain). These data, along with our partial 2-DE maps of S. iniae ATCC29178 and L. garvieae KG9408, may help suggest antigenic proteins for the development of effective diagnostic tools and vaccines against S. iniae and L. garvieae.

KEY WORDS: Streptococcus iniae · Lactococcus garvieae · Two-dimensional gel electrophoresis · Mass spectrometry · Proteome

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