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Diseases of Aquatic Organisms

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DAO 71:19-24 (2006)  -  doi:10.3354/dao071019

Efficacy of a Listonella anguillarum (syn. Vibrio anguillarum) vaccine for juvenile sea bass Dicentrarchus labrax

Panagiotis Angelidis1,*, Dimitrios Karagiannis1, Elizabeth M. Crump2

1Laboratory of Ichthyology and Ichthyopathology, Aristotle University of Thessaloniki, PO Box 395, 54124 Thessaloniki, Greece
2Microtek International, 6761 Kirkpatrick Crescent, Saanichton, British Columbia V8M 1Z8, Canada

ABSTRACT: The efficacy of a commercial bivalent Listonella anguillarum (serotype 01 and 02) vaccine (MICROViB, Microtek International) was tested on prime- and booster-immersion vaccinated sea bass Dicentrarchus labrax juveniles. We carried out 2 challenge tests on the prime-vaccinated fish, 50 and 90 d after initial vaccination. A second group of fish received a booster vaccination 60 d after the prime vaccination, and were tested with a single challenge 30 d later. Relative percent survival (RPS) was 92 and 84% (both p < 0.01) among the prime-vaccinated fish on the first and second challenges, respectively. The RPS of the booster-vaccinated sea bass was 100% (p < 0.01). Antibody titres were tested only among 10 prime-vaccinated and 10 unvaccinated (control) sea bass, 60 d post-immunisation, and were found to rise to 1/32 in the vaccinated fish. Our results demonstrate that MICROViB immersion vaccine can effectively protect juvenile sea bass from L. anguillarum infection.

KEY WORDS: Immersion · Vaccination · Dicentrarchus labrax · Vibrio anguillarum · Listonella anguillarum · Vibriosis

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