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Diseases of Aquatic Organisms

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DAO 71:81-85 (2006)  -  doi:10.3354/dao071081

Molecular characterisation of an Australian isolate of Bonamia exitiosa

Serge Corbeil1,*, Isabelle Arzul2, Maeva Robert2, Franck C. J. Berthe3, Nathalie Besnard-Cochennec4, Mark St. J. Crane1

1AAHL Fish Diseases Laboratory, Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia
2IFREMER Laboratoire Génétique et Pathologie, Ronce-les-bains BP 133, 17390 La Tremblade, France
3Department of Pathology & Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Canada
4IFREMER, BP7004, Taravao, Tahiti, French Polynesia

ABSTRACT: An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.

KEY WORDS: Bonamia species · Ostrea angasi · Flat oyster · Small subunit rRNA operon

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