Inter-Research > DAO > v72 > n2 > p93-99  
DAO
Diseases of Aquatic Organisms

via Mailchimp

DAO 72:93-99 (2006)  -  doi:10.3354/dao072093

Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening

Li Yuan1,2, Xiaohua Zhang1, Nan Xiao1,2, Lingfen Dai1, Weizhou Chen3, Chensong Jia1, Ruohong Zhao4, Sean M. Hemmingsen4, Heping Dai1,*

1Institute of Hydrobiology, Chinese Academy of Sciences, 7 East Lake Southern Road, Wuhan 430072, PR China
2Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China
3Marine Biology Laboratory Nanao Station, Key Laboratory of Marine Biology of Guangdong Province, Shantou University, Shantou 515900, PR China
4Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, Saskatoon S7N 0W9, Canada
*Corresponding author. Email:

ABSTRACT: White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.


KEY WORDS: White spot syndrome virus (WSSV) · Phage display · Neutralizing antibody · Single chain fragment variable regions (scFv) antibody


Full text in pdf format
Next article