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Diseases of Aquatic Organisms

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DAO 73:201-205 (2007)  -  doi:10.3354/dao073201

Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae

Audun H. Nerland1,*, Cecilie Skaar1, Tove Boge Eriksen1, Hogne Bleie2

1Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway
2National Veterinary Institute, PO Box 1263 Sentrum, 5811 Bergen, Norway

ABSTRACT: We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 104 TCID50 (50% tissue culture infectious dose) ml–1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 107 virus particles ml–1 seawater.

KEY WORDS: Nodavirus · RT-PCR · Real-time PCR · Quantification · Seawater · Atlantic halibut

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