DAO 74:171-178 (2007)  -  doi:10.3354/dao074171

White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP)

Li-Li Chen1,*, Li-Chen Lu1, Wan-Jung Wu1, Chu-Fang Lo2, Wei-Pang Huang2

1Department of Biomedical Sciences, Chung Shan Medical University, No. 110, Sec. 1, Chien-Kuo N. Road, Taichung 402, Taiwan, ROC
2Institute of Zoology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan 10617, ROC

ABSTRACT: White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. In order to further explore the link between WSSV067 and host proteins, we performed a yeast 2-hybrid screening of a Penaeus monodon cDNA library, using WSSV067C as bait. One of the molecules that specifically interacted with WSSV067C was the P. monodon chitin-binding protein (PmCBP). An in vitro binding assay showed that c-myc-WSSV067C was capable of co-precipitating HA-PmCBP-C. Furthermore, PmCBP was expressed in almost all organs but appeared to be up-regulated at the late stage of WSSV infection.

KEY WORDS: Penaeus monodon · White spot syndrome virus · VP53A · Envelope protein ·Chitin-binding protein

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