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Diseases of Aquatic Organisms

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DAO 77:175-179 (2007)  -  DOI:

Prevalence of white spot syndrome virus (WSSV) in wild shrimp Penaeus monodon in the Philippines

Leobert D. de la Peña*, Celia R. Lavilla-Pitogo, Corina Belle R. Villar, Milagros G. Paner, Christopher D. Sombito, Geimbo C. Capulos

Southeast Asian Fisheries Development Center, Aquaculture Department, Tigbauan 5021, Iloilo, Philippines

ABSTRACT: Prevalence of white spot syndrome virus (WSSV) was determined using polymerase chain reaction (PCR) methodology on DNA extracted from the gills of wild black tiger shrimp Penaeus monodon collected from 7 sampling sites in the Philippines. These 7 sampling sites are the primary sources of spawners and broodstock for hatchery use. During the dry season, WSSV was detected in shrimp from all sites except Bohol, but during the wet season it was not detected in any site except Palawan. None of the WSSV-PCR positive shrimp showed signs of white spots in the cuticle. Prevalence of WSSV showed seasonal variations, i.e. prevalence in dry season (April to May) was higher than in the wet season (August to October). These results suggest that WSSV has already become established in the local marine environment and in wild populations of P. monodon. Thus, broodstock collected during the dry season could serve as the main source of WSSV contamination in shrimp farms due to vertical transmission of the virus in hatcheries.

KEY WORDS: Disease prevalence · WSSV · Wild Penaeus monodon · PCR

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Cite this article as: de la Peña LD, Lavilla-Pitogo CR, Villar CBR, Paner MG, Sombito CD, Capulos GC (2007) Prevalence of white spot syndrome virus (WSSV) in wild shrimp Penaeus monodon in the Philippines. Dis Aquat Org 77:175-179.

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