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Diseases of Aquatic Organisms

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DAO 77:225-233 (2007)  -  DOI:

Ultrastructure of sporulation in Haplosporidium armoricanum

P. M. Hine1,*, M. Y. Engelsma2, St. J. Wakefield3

1Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, PO Box 40-742, Upper Hutt 6007, New Zealand
2CIDC-Lelystad, Central Institute for Animal Disease Control, PO Box 2004, 8203 AA Lelystad, The Netherlands
3Wellington School of Medicine, University of Otago, Private Bag 7902, Wellington South, New Zealand

ABSTRACT: An ultrastructural study was carried out on the tissues of an oyster (Ostrea edulis), heavily infected with Haplosporidium armoricanum (van Banning, 1977), that had been fixed in Carson’s fixative. The well-fixed tissues revealed details of sporulation and of the spores, which had not been previously reported from H. armoricanum. These include the initial presence of sparse haplosporosomes after thickening of the plasma membrane in early sporonts, division of sporont nuclei by multiple fission, cup-like indentations in the nuclear surface associated with putative nuclear material in both the sporonts and spores, and cytoplasmic multi-vesicular bodies in the cytoplasm of sporonts and spores. The spore wall and operculum were formed from a light matrix that occurred in short cisternae of smooth endoplasmic reticulum in the episporoplasm, and parallel bundles of microfibrils were present in some spores. Spores were rarely bi-nucleate with the nuclei occurring as a diplokaryon, with putative nuclear material at the junction of the 2 nuclei. Nuclear membrane-bound Golgi (NM-BG) cisternae were common in spores, and they appeared to synthesise a light granular material into lysosome-like granules. Dense bodies similar to those reported from H. lusitanicum, H. pickfordi and H. monforti occurred in, or outside, the peripheral endosporoplasm, which was closely apposed to the spore wall. Spore haplosporosomes were frequently axehead-shaped, more like those of H. costale than those previously reported from H. armoricanum, and in some haplosporosomes there was a small round lucent patch with a dark point near the centre of the lucent patch. Overall, H. armoricanum appears to be closely related to H. costale and Bonamia spp. Although the endosporoplasm of H. armoricanum has NM-BG and it resembles the uni-nucleate stage, it appears to be unlikely that they are the same, as the axehead-shaped haplosporosomes of the spore differ considerably from the spherical haplosporosomes of vegetative stages.

KEY WORDS: Haplosporidium armoricanum · Ultrastructure · Sporulation · Golgi · Haplosporogenesis

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Cite this article as: Hine PM, Engelsma MY, Wakefield SJ (2007) Ultrastructure of sporulation in Haplosporidium armoricanum. Dis Aquat Org 77:225-233.

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