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Diseases of Aquatic Organisms

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DAO 79:27-35 (2008)  -  DOI:

Antigenic and molecular characterization of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chile

Andrés Silva-Rubio1, Claudia Acevedo1, Beatriz Magariños2, Beltrán Jaureguiberry1, Alicia E. Toranzo2, Ruben Avendaño-Herrera1,*

1Laboratorio de Veterquímica, Camino a Melipilla 5641, Cerrillos, Santiago, Chile
2Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain
*Corresponding author. Email:

ABSTRACT: Biochemical, serological and molecular properties of a group of 14 Vibrio ordalii strains isolated from cultured Atlantic salmon Salmo salar in Chile in recent years were studied. The characteristics of isolates were compared with the type strain V. ordalii ATCC 33509T. The Chilean V. ordalii represented a biochemically homogenous group; however, some minor differences with the type strain were observed. The serological relationships among isolates, as well as the study of their antigenic determinant (LPS) revealed a strong reaction with antisera raised against Atlantic salmon strains and the antiserum raised against Listonella anguillarum serotype O2. However, LPS electrophoretic patterns were completely different from the V. ordalii type strain, regardless of the serum employed, suggesting the possibility that the Chilean strains constitute a new serological subgroup within this bacterial species. Genetic analyses by PFGE, RAPD, REP-PCR and ERIC-PCR demonstrated that all V. ordalii strains were genetically homogenous, displaying similar DNA patterns, regardless of the techniques used. Moreover, the analysis of DNA banding patterns generated by ERIC-PCR and REP-PCR also clearly separated the type strain from the Chilean strains. This is the first report of characterization of V. ordalii strains from the Southeastern Pacific area, the results of which should facilitate the development of vaccines for protecting cultured Atlantic salmon against vibriosis in this area.

KEY WORDS: Vibrio ordalii · Fish vibriosis · Serotyping · Genotyping · Atlantic salmon

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Cite this article as: Silva-Rubio A, Acevedo C, Magariños B, Jaureguiberry B, Toranzo AE, Avendaño-Herrera R (2008) Antigenic and molecular characterization of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chile. Dis Aquat Org 79:27-35.

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