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Diseases of Aquatic Organisms

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DAO 79:141-145 (2008)  -  DOI:

Natural aquatic insect carriers of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV)

R. Sudhakaran1, P. Haribabu2, S. Rajesh Kumar1, M. Sarathi1, V. P. Ishaq Ahmed1, V. Sarath Babu1, C. Venkatesan1, A. S. Sahul Hameed1,*

1Aquaculture Biotechnology Division, Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore Dt., Tamil Nadu, India
2Sri Venkateswara Veterinary University, Department of Aquaculture, College of Fishery Science, Muthukur 524 344, Andhra Pradesh, India
*Corresponding author. Email:

ABSTRACT: Five different species of aquatic insects were collected from nursery ponds containing the freshwater prawn Macrobrachium rosenbergii infected with Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). The insects were screened as potential natural carriers of MrNV and XSV. RT-PCR (reverse transcription polymerase chain reaction) analysis gave positive results for MrNV and XSV in Belostoma sp., Aesohna sp., Cybister sp. and Notonecta sp., and negative results for Nepa sp. An Aedes albopictus mosquito cell line (C6/36) was used for infectivity assays, with viral inoculum prepared from the aquatic insects, since C6/36 cells have recently been shown to be susceptible to infection with MrNV and XSV. The C6/36 cells were harvested 4 d post-challenge for examination by electron microscopy. This revealed aggregation of viral particles throughout the cytoplasm for cells challenged with inocula from all the insect species except Nepa sp. Our results indicate that several aquatic insect species may present a risk for MrNV and XSV transmission to M. rosenbergii.

KEY WORDS: Macrobrachium rosenbergii · Aquatic insects · Macrobrachium rosenbergii nodavirus · MrNV · Extra small virus · XSV · Natural hosts · RT-PCR

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Cite this article as: Sudhakaran R, Haribabu P, Rajesh Kumar S, Sarathi M and others (2008) Natural aquatic insect carriers of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). Dis Aquat Org 79:141-145.

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