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Diseases of Aquatic Organisms

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DAO 81:153-161 (2008)  -  DOI:

Vibrio tapetis-like strain isolated from introduced Manila clams Ruditapes philippinarum showing symptoms of brown ring disease in Norway

Christine Paillard1,*, Kjetil Korsnes2,3, Patrick Le Chevalier4, Christine Le Boulay4, Lisbeth Harkestad2, Anne Grete Eriksen2, Endre Willassen5, Øivind Bergh2,6, Clément Bovo1, Cecilie Skår2, Stein Mortensen2

1Institut Universitaire Européen de la Mer (IUEM). LEMAR. UMR 6539. Université de Bretagne Occidentale,
29280 Plouzané, France
2Institute of Marine Research, PO Box 1870, Nordnes, 5817 Bergen, Norway
3Bodø University College, 8049 Bodø, Norway
4Laboratoire Universitaire de Microbiologie Appliquée de Quimper (LUMAQ) IUT de génie biologique, Université de Bretagne Occidentale, 29334 Quimper, France
5The Natural History Collections, and 6Department of Biology, PO Box 7800, 5020 Bergen, Norway

ABSTRACT: The Manila clam Ruditapes philippinarum was introduced to Norway in 1987 and was produced in 2 hatcheries until 1991. Clam seed was planted at 6 sites. Two sites were on the Island of Tysnes, south of Bergen. Surviving adult Manila clams were recovered in 1995 and 1996. In the present study, Manila clams from the original seeding that displayed morphological signs of brown ring disease (BRD) were recovered in June 2003 (n = 7) and in June 2004 (n = 17). Samples from extrapallial fluid, tissues and haemolymph were inoculated on marine agar. Replicate subcultures on selective media were used to select potential Vibrio tapetis strains, and in total, 190 bacterial strains were isolated. One of these strains clustered within the V. tapetis clade and was named NRP 45. DNA:DNA hybridisation with the type strain CECT4600 showed 52.7 and 57.3% DNA:DNA similarity. Hybridisation of NRP 45 and the V. tapetis LP2 strain, isolated from corkwing wrasse Symphodus melops, produced 46.6 and 44.4% re-association. Partial gene segments encoding 16S rRNA, gyrase B protein (GyrB) and chaperonin 60 protein (Cpn60) were characterised and compared to CECT 4600. NRP 45 showed 5 differences in the 1416 nucleotides (nt) of the 16S rRNA encoding gene (99.6% similarity), while the GyrB encoding gene had 62 substitutions of 1181 nt compared (94.8% similarity) and the Cpn60 encoding gene had 22 substitutions out of 548 nt compared (96% similarity). This is the first finding of BRD and the first isolation of a V. tapetis-like bacterial strain from a bivalve in Norway.

KEY WORDS: Brown ring disease · Vibrio tapetis · Bivalve · Manila clams · Bacteria · Norway

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Cite this article as: Paillard C, Korsnes K, Le Chevalier P, Le Boulay C and others (2008) Vibrio tapetis-like strain isolated from introduced Manila clams Ruditapes philippinarum showing symptoms of brown ring disease in Norway. Dis Aquat Org 81:153-161.

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