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Diseases of Aquatic Organisms

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DAO 82:45-54 (2008)  -  DOI:

Distribution and genetic characterization of Mycobacterium chelonae in laboratory zebrafish Danio rerio

Christopher M. Whipps1,*, Jennifer L. Matthews2, Michael L. Kent3

1State University of New York College of Environmental Science and Forestry, Environmental and Forest Biology,
246 Illick Hall, 1 Forestry Drive, Syracuse, New York 13210, USA
2Zebrafish International Resource Center, 5274 University of Oregon, Eugene, Oregon 97403-5274, USA
3Center for Fish Disease Research, Department of Microbiology, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331-3404, USA

ABSTRACT: During routine screening of zebrafish at a research facility, histological changes consistent with mycobacteriosis were observed, prompting an investigation to determine the background prevalence and distribution of Mycobacterium species throughout the facility. Infection status was evaluated in 240 zebrafish representing 9 genetic lines, using histology, culture and PCR. Environmental sources were also tested for the presence of mycobacteria. Prevalence in zebrafish by culture and PCR was 10% (24/240), 21 of which were TU line fish. All isolates from fish were identified as M. chelonae by hsp65 DNA sequencing; subsequent DNA fingerprinting delineated 3 strains, designated H1E1 (1/24), H1E2 (22/24), and H1E3 (1/24). From external sources, tank or tubing surface biofilms were positive by culture (13/32) with multiple species and strains isolated including M. neoaurum, M. phocaicum, and identical strains of M. chelonae that were found in zebrafish: H1E1 (2/13) and H1E2 (8/13). Comparing diagnostic methods, acid-fast stained histological sections showed substantial agreement with plate culture and PCR for detection of mycobacteria in fish. Observation of granulomas in hematoxylin and eosin-stained sections was a less reliable predictor of mycobacteriosis, as uninfected females with egg-associated inflammation and hyperplasia were misdiagnosed. These data revealed background levels of mycobacteriosis in a healthy and well-managed facility. Infected populations were removed, although the apparent ability for M. chelonae to remain viable in environmental reservoirs may make it difficult to eradicate completely. This highlights the importance of an animal-health monitoring program and good husbandry practices to prevent disease in zebrafish research laboratories.

KEY WORDS: Mycobacterium chelonae · Zebrafish · Danio rerio · Biofilms · Enterobacterial repetitive intergenic consensus PCR · ERIC-PCR · Randomly amplified polymorphic DNA · RAPD

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Cite this article as: Whipps CM, Matthews JL, Kent ML (2008) Distribution and genetic characterization of Mycobacterium chelonae in laboratory zebrafish Danio rerio. Dis Aquat Org 82:45-54.

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