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Diseases of Aquatic Organisms

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DAO 83:97-103 (2009)  -  DOI:

B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish

Kjersti B. Mézeth1,*, Sonal Patel1,2, Håvard Henriksen1, Anne Marie Szilvay1, Audun H. Nerland1,2,3

1Department of Molecular Biology, University of Bergen, Postbox 7803, 5020 Bergen, Norway
2Institute for Marine Research, Postbox 1870 Nordnes, 5817 Bergen, Norway
3Present address: The Gade Institute, University of Bergen, 5021 Bergen, Norway

ABSTRACT: Betanodavirus infects both larvae and juvenile fish and can cause the disease viral encephalopathy and retinopathy (VER). During an acute outbreak of VER, infected individuals display several clinical signs of infection, i.e. abnormal swimming pattern and loss of appetite. Betanodaviruses can also cause chronic or persistent infection where the infected individuals show no clinical signs of infection. During infection the viral sub-genomic RNA3 and the RNA3-encoded B2 protein are expressed. Antibodies against the B2 protein from Atlantic halibut nodavirus were raised and used together with antibodies against the capsid protein to detect the presence of these 2 viral proteins in infected cells in culture and at different stages of infection in Atlantic halibut Hippoglossus hippoglossus and Atlantic cod Gadus morhua. The B2 protein was detected in recently infected, but not in chronically infected fish. Results suggest that the detection of B2 may be used to discriminate a recent and presumably active infection from a chronic and presumably persistent infection.

KEY WORDS: Fish nodavirus · Atlantic halibut · Atlantic cod · B2 protein · Viral nervous necrosis

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Cite this article as: Mézeth KB, Patel S, Henriksen H, Szilvay AM, Nerland AH (2009) B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish. Dis Aquat Org 83:97-103.

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