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Diseases of Aquatic Organisms

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DAO 84:195-200 (2009)  -  DOI:

Detection of Laem-Singh virus in cultured Penaeus monodon shrimp from several sites in the Indo-Pacific region

Nusra Sittidilokratna1, 2, 6, Sirintip Dangtip2, Kallaya Sritunyalucksana1,2, Ravi Babu3, Balakrishnan Pradeep4, C. V. Mohan5, Nicholas Gudkovs6, Peter J. Walker6,*

1National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Phathumthani 12120, Thailand
2Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Phyathai, Bangkok 10400, Thailand
3National Centre for Sustainable Aquaculture, 69-17-8, SBI Officers Colony, Rajendra Nagar, Kakinada 533003, Andhra Pradesh, India
4Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, India
5Network of Aquaculture Centers Asia-Pacific (NACA), Suraswadi Building, Kasetsart University Campus, Ladyao, Jatujak, Bangkok 10900, Thailand
6CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL), 5 Port Arlington Road, Geelong, Victoria 3220, Australia
*Corresponding author. Email:

ABSTRACT: Laem-Singh virus (LSNV) is a positive-sense single-stranded RNA (ssRNA) virus that was recently identified in Penaeus monodon shrimp in Thailand displaying signs of slow growth syndrome. A total of 326 shrimp collected between 1998 and 2007 from countries in the Indo-Pacific region were tested by RT-PCR for evidence of LSNV infection. The samples comprised batches of whole postlarvae, and lymphoid organ, gill, muscle or pleopod tissue of juvenile, subadult and adult shrimp. LSNV was not detected in 96 P. monodon, P. japonicus or P. merguiensis from Australia or 16 P. monodon from Fiji, Philippines, Sri Lanka and Mozambique. There was no evidence of LSNV infection in 73 healthy juvenile P. vannamei collected during 2006 from ponds at 9 locations in Thailand. However, LNSV was detected in each of 6 healthy P. monodon tested from Malaysia and Indonesia, 2 of 6 healthy P. monodon tested from Vietnam and 39 of 40 P. monodon collected from slow-growth ponds in Thailand. A survey of 81 P. monodon collected in 2007 from Andhra Pradesh, India, indicated 56.8% prevalence of LSNV infection but no clear association with disease or slow growth. Phylogenetic analysis of PCR amplicons obtained from samples from India, Vietnam, Malaysia and Thailand indicated that nucleotide sequence variation was very low (>98% identity) and there was no clustering of viruses according to site of isolation or the health status of the shrimp. The data suggests that LSNV exists as a single genetic lineage and occurs commonly in healthy P. monodon in parts of Asia.

KEY WORDS: Laem Singh virus · Shrimp · Geographic distribution

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Cite this article as: Sittidilokratna N, Dangtip S, Sritunyalucksana K, Babu R and others (2009) Detection of Laem-Singh virus in cultured Penaeus monodon shrimp from several sites in the Indo-Pacific region. Dis Aquat Org 84:195-200.

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