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Diseases of Aquatic Organisms

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DAO 85:147-155 (2009)  -  DOI:

Complete life cycle of Myxobolus rotundus (Myxosporea: Myxobolidae), a gill myxozoan of common bream Abramis brama

Cs. Székely1,*, S. L. Hallett2, S. D. Atkinson2, K. Molnár1

1Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581 Budapest, Hungary
2Department of Microbiology, Oregon State University, Corvallis, Oregon 97331, USA

ABSTRACT: The life cycle of Myxobolus rotundus Nemeczek, 1911, a myxosporean parasite of the gills of common bream Abramis brama L., was studied under laboratory conditions. Mature Myxobolus spp. spores from plasmodia in the gills of wild bream were used to infect naïve oligochaete worms in a flow-through system of aquaria. Triactinomyxon-type actinospores were released from the oligochaetes 1 yr later and allowed to continually flow into a tank containing uninfected bream fry. The gills of the fry were checked for development of plasmodia in squash preparations 3 d post-exposure, and then at weekly intervals for 8 wk. Tissue samples were fixed at each time point. Developing plasmodia were first observed 17 d post-exposure (Day 17). Mature spores were collected from plasmodia on Day 56 and were added to plastic dishes containing parasite-free Tubifex tubifex oligochaetes. Second-generation actinospores were released from these worms 8 mo post-exposure, and were morphologically identical to first-generation spores. Myxospores obtained from the bream fry were morphologically identical to those identified in wild bream as M. rotundus. Small subunit ribosomal RNA gene sequences obtained from first- and second-generation actinospores and the bream fry myxospores were 100% similar to M. rotundus spores from the original wild fish.

KEY WORDS: Myxobolus rotundus · Myxospore · Gill parasite · Abramis brama · Triactinomyxon · Tubifex tubifex · 18S rDNA · Lake Balaton

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Cite this article as: Székely Cs, Hallett SL, Atkinson SD, Molnár K (2009) Complete life cycle of Myxobolus rotundus (Myxosporea: Myxobolidae), a gill myxozoan of common bream Abramis brama. Dis Aquat Org 85:147-155.

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