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Diseases of Aquatic Organisms

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DAO 87:151-160 (2009)  -  DOI:

Phylogenetic analysis of betanodavirus isolates from Australian finfish

N. J. G. Moody1,3,*, P. F. Horwood1, A. Reynolds1, T. J. Mahony2, I. G. Anderson1, H. J. Oakey1

1Tropical and Aquatic Animal Health Laboratory, Department of Primary Industries and Fisheries,
180-202 River Boulevard, Townsville, Queensland 4810, Australia
2Molecular Biosciences Technologies, Department of Primary Industries and Fisheries,
306 Carmody Road, St Lucia, Queensland 4067, Australia
3Present address: Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia

ABSTRACT: In Australia, disease caused by betanodavirus has been reported in an increasing number of cultured finfish since the first report of mortalities in 1990. Partial coat protein gene sequences from the T2 or T4 regions of 8 betanodaviruses from barramundi Lates calcarifer, sleepy cod Oxyeleotris lineolata, striped trumpeter Latris lineata, barramundi cod Cromileptes altivelis, Australian bass Macquaria novemaculata and gold-spotted rockcod Epinephelus coioides from several Australian states were determined. Analysis of the 606 bp nucleotide sequences of the T2 region of 4 isolates demonstrated the close relationship with isolates from the red-spotted grouper nervous necrosis virus (RGNNV) genotype and the Cluster Ia subtype. Comparison of a smaller 289 bp sequence from the T4 region identified 2 distinct groupings of the Australian isolates within the RGNNV genotype. Isolates from barramundi from the Northern Territory, barramundi, sleepy cod, barramundi cod and gold-spotted rockcod from Queensland, and striped trumpeter from Tasmania shared a 96.2 to 99.7% nucleotide identity with each other. These isolates were most similar to the RGNNV genotype Cluster Ia. Isolates from Australian bass from New South Wales and from barramundi from South Australia shared a 98.6% sequence identity with each other. However, these isolates only shared an 85.8 to 87.9% identity with the other Australian isolates and representative RGNNV isolates. The closest nucleotide identity to sequences reported in the literature for the New South Wales and South Australian isolates was to an Australian barramundi isolate (Ba94Aus) from 1994. These 2 Australian isolates formed a new subtype within the RGNNV genotype, which is designated as Cluster Ic.

KEY WORDS: Betanodavirus · Phylogeny · Nervous necrosis virus · Coat protein · Nodavirus · Molecular detection

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Cite this article as: Moody NJG, Horwood PF, Reynolds A, Mahony TJ, Anderson IG, Oakey HJ (2009) Phylogenetic analysis of betanodavirus isolates from Australian finfish. Dis Aquat Org 87:151-160.

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