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Endangered Species Research

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ESR 9:133-142 (2009)  -  DOI:

First generation microarray-system for identification of primate species subject to bushmeat trade

A.-C. Rönn1, O. Andrés2, F. López-Giráldez2,3, C. Johnsson-Glans1, E. J. Verschoor4, X. Domingo-Roura2,†, M. W. Bruford5, A.-C. Syvänen1, M. Bosch2,*

1Molecular Medicine, Department of Medical Sciences, Uppsala University, University Hospital, Entr. 70, 3rd floor, Res. Dep. 2, 75185 Uppsala, Sweden
2Genètica de la Conservació Animal, Institut de Recerca i Tecnologia Agroalimentàries, Carretera de Cabrils Km2, 08348 Cabrils, Spain
3Department of Ecology and Evolutionary Biology, Yale University, PO Box 208106, Prospect Street 165, New Haven, Connecticut 06520, USA
4Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288 GJ Rijswijk, The Netherlands
5Cardiff School of Biosciences, Cardiff University, PO Box 915 Cathays Park, Cardiff CF10 3TL, UK
*Corresponding author: Email: Deceased

ABSTRACT: About a quarter of non-human primate species are threatened by extinction in the near future. Loss of habitat, disease and illegal hunting, especially for the bushmeat trade, are major causes of concern. Here, we develop an identification tool for primate genera using diagnostic nucleotide positions in the epsilon globin gene, apolipoprotein B gene and mitochondrial 12S rRNA. We identified 111 diagnostic nucleotide positions suitable for genotyping by a minisequencing assay in a microarray format. To show the applicability of the microarray, we typed 70 non-human primates representing all primate infraorders. Sixty-five samples were assigned to the correct infraorder, and 32 were assigned to the correct genus (the highest level of taxonomic resolution attempted here). Our results show that it is feasible to distinguish among a high number of primate taxa if the system allows hierarchical assignation of the samples at different taxonomic levels and includes both taxon-specific and redundant positions.

KEY WORDS: Primates · Bushmeat · Minisequencing microarray · Epsilon globin · Apolipoprotein B · 12S rRNA

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Cite this article as: Rönn AC, Andrés O, López-Giráldez F, Johnsson-Glans C and others (2009) First generation microarray-system for identification of primate species subject to bushmeat trade. Endang Species Res 9:133-142.

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