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Marine Ecology Progress Series

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MEPS 121:53-63 (1995)  -  doi:10.3354/meps121053

Influence of zooplankton grazing on free dissolved enzymes in the sea

Bochdansky AB, Puskaric S, Herndl GJ

In the Northern Adriatic Sea, extracellular enzymatic activity was measured during a Lagrangian study following a drifting buoy for 40 h. Dissolved free enzymatic activity represented 20 to 70% of total activity depending on the type of enzyme. alpha- and beta-glucosidases exhibited a significantly higher free activity than proteolytic enzymes. In subsequent laboratory experiments we investigated the effect of zooplankton on the free enzyme pool. The 4-step approach included: (1) determination of the enzymatic activities in copepods (mainly Acartia clausi); (2) enzymatic activity in fecal pellets; (3) short- and long-term grazing experiments; and (4) degradability of free glucosidase in seawater. alpha- and beta-glucosidases, leu-aminopeptidase, lipase and chitinase were examined. Experiments in which zooplankton were selectively enriched revealed a significant increase in both particle-bound (due to the increase of bacterial density) and dissolved free enzymatic activity. Incubating water enriched in free enzymes released by zooplankton with natural bacterial consortia, we found that 70% of the original alpha- and beta-glucosidase activity remained after 22 h. The presence of microorganisms did not enhance the degradation of these enzymes as compared to autoclaved controls. We found that a considerable amount of free dissolved enzymes is lost by 0.2 um filtration using Nuclepore filters, thereby leading to an underestimation of dissolved enzymes by ~30% in our experiments. Based on our results we conclude that mesozooplankton contribute to the free enzymatic activity in natural waters especially during periods of high grazing activity.

Extracellular enzymes . Zooplankton . Dissolved enzymes . Fecal pellet . Northern Adriatic Sea

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