Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow; D. G. Cummings; S. W. Gibb

doi:10.3354/meps161303

MEPS

Marine Ecology Progress Series

Online: ISSN 1616-1599

Print: ISSN 0171-8630

DOI: https://doi.org/10.3354/meps

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MEPS is a leading hybrid research journal on all aspects of marine, coastal and estuarine ecology. Priority is given to outstanding research that advances our ecological understanding.

Online: ISSN 1616-1599

Print: ISSN 0171-8630

DOI: https://doi.org/10.3354/meps

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Mar Ecol Prog Ser 161:303-307 (1997)

DOI: https://doi.org/10.3354/meps161303

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Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow
D. G. Cummings
S. W. Gibb

ABSTRACT: A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil^® MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoidpigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides anoptimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.

KEYWORDS

HPLC C-8 column Improved resolution Chlorophylls Carotenoids

R. G. Barlow (Co-author)

D. G. Cummings (Co-author)

S. W. Gibb (Co-author)

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