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MEPS 219:85-98 (2001)  -  doi:10.3354/meps219085

Chlorophyll c2 monogalactosyldiacylglyceride ester (chl c2-MGDG). A novel marker pigment for Chrysochromulina species (Haptophyta)

Manuel Zapata1,2,*, Bente Edvardsen3, Francisco Rodríguez1, Miguel A. Maestro4, José L. Garrido5

1CIMA, Consellería de Pesca, Xunta de Galicia, Apdo. 13, 36620 Vilanova de Arousa, Spain
2Departamento de Bioloxía Vexetal, Universidade de Vigo, 36200 Vigo, Spain
3Section of Marine Botany, Department of Biology, University of Oslo, PO Box 1069, 0316 Oslo, Norway
4Servicios Xerais de Apoio a Investigación, Universidade da Coruña, 15071 A Coruña, Spain
5Instituto de Investigacións Mariñas, CSIC, Av. Eduardo Cabello 6, 36208 Vigo, Spain

ABSTRACT: The pigment composition of the haptophyte Chrysochromulina polylepis (Strain CCMP 286) was analysed by high-performance liquid chromatography (HPLC) using pyridine-containing mobile phases and polymeric C18, or monomeric C8 columns. The polar chlorophyll (chl) c pigment composition included chl c2 and chl c3 as major fractions, and divinyl protochlorophyllide a (DV pchlide or MgDVP) as a minor component. Several non-polar fluorescent peaks sharing a common chl c-type absorption spectrum were also detected. The main component of these peaks was isolated and characterised by chromatographic behaviour, UV-visible (UV-VIS) and fluorescence spectroscopy. Although spectral properties were similar to a high molecular weight non-polar chl c (1313 Da) recently characterised from Emiliania huxleyi, both chlorophylls showed different chromatographic behaviour. Fast atom bombardment-mass spectrometry (FAB-MS) analysis showed a high mass molecular ion (m/z 1265), and a fragmentation pattern compatible with a molecular structure consisting of a chl c2 pigment linked by an ester bond to the sugar moiety of a monogalactosyl diacylglyceride (MGDG), which included 2 myristic acid (14:0) residues. To study the distribution pattern of the novel pigment, different strains of C. polylepis and other species of the genus Chrysochromulina were analysed. A similar chl c2 ester was also present in 2 C. polylepis strains (K, B11) and in C. aff. polylepis (PLY 200), as well as in C. acantha, C. camella, C. leadbeateri, C. strobilus, C. throndsenii, and Chrysochromulina sp. (CS-410). Three species, C. fragaria, C. hirta (2 strains) and C. kappa, lacked the new chl c, but they contained detectable amounts of other non-polar chl c-like pigments. The feasibility of using chls c and carotenoids as marker pigments to detect Chrysochromulina species in field samples is discussed.

KEY WORDS: Chlorophyll c2-galactolipid esters · Chrysochromulina polylepis · Chrysochromulina spp. · Fast atom bombardment-mass spectrometry · HPLC pigment analysis · Marker pigments · Chemotaxonomy

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