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Marine Ecology Progress Series

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MEPS 235:205-216 (2002)  -  doi:10.3354/meps235205

Assessment of coastal pollution by combined determination of chemical and biochemical markers in Mullus barbatus

Cinta Porte*, Estefania Escartín, Luz M. García de la Parra**, Xavier Biosca, Joan Albaigés

Department of Environmental Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain
*E-mail: **Present address: Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán en Acuicultura y Manejo Ambiental, Avenida Sábalo-Cerritos s/n, 82010 Mazatlán, Sinaloa, Mexico

ABSTRACT: Muscle concentrations of organochlorinated compounds as well as biliary levels of polycyclic aromatic hydrocarbon (PAH) metabolites were determined in red mullet Mullus barbatus as a sentinel species for assessing the pollution along the western Mediterranean coast. A battery of biochemical markers‹biotransformation and antioxidant enzymes‹were also measured in liver subcellular fractions to assess exposure to pollutants. Among them, 7-ethoxyresorufin O-deethylase (EROD) activity, as well as 7-penthoxyresorufin O-deethylase (PROD) activity and cytochrome P450 1A (CYP1A) content evidenced strong differences among sampling sites, and a good correlation with the amount of PCBs bioaccumulated by fish. No clear pollution-related response was observed for cytosolic glutathione S-transferase, whereas uridine-diphosphate UDP-glucuronyltransferase (UDPGT) was elevated in fish from polluted sites. Antioxidant enzymes‹superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX)‹were used to assess oxidative stress. Among them, catalase activity was well related to PCB body burden.

KEY WORDS: Cytochrome P450 system · Antioxidant enzymes · Biomonitoring · Mullus barbatus

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