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Marine Ecology Progress Series

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MEPS 256:57-62 (2003)  -  doi:10.3354/meps256057

HR MAS 1H NMR spectroscopy analysis of marine microalgal whole cells

Matilde S. Chauton1,*, Odd Inge Optun2, Tone F. Bathen2, Zsolt Volent3, Ingrid S. Gribbestad2, Geir Johnsen1

1Norwegian University of Science and Technology, Trondhjem Biological Station, 7491 Trondheim, Norway
2Sintef Unimed MR-centre, 7465 Trondheim, Norway
3Sintef Fisheries and Aquaculture, 7465 Trondheim, Norway

ABSTRACT: To study the use of nuclear magnetic resonance (NMR) spectroscopy as a method of classification, we performed high-resolution magic angle spinning proton (HR MAS 1H) NMR spectroscopy analysis of whole-cell samples of Dunaliella sp. (Chlorophyceae), Amphidinium carterae (Dinophyceae), Phaeodactylum tricornutum and Thalassiosira pseudonana (Bacillariophyceae). Emphasising the potential use of NMR spectroscopy as a routine analysis of microalgae we chose a straightforward procedure for culturing and harvesting, without extraction or radioactive labelling. We obtained well-resolved HR MAS 1H NMR spectra from the 4 algae, despite the fact that our samples contained whole cells and some residual sea water. Selected parts of 5 replicate spectra from each microalga were used as input in 2 multivariate pattern-recognition strategies (principal component analysis and fuzzy clustering), both analyses showing clear grouping of the different species. Two spectra from a previous sample run (cultures grown under the same conditions) were also included, and both were correctly classified. We therefore consider HR MAS 1H NMR spectroscopy to be a potential method of classification for microalgae, with statistical data processing indicating replicability and robustness of the method.

KEY WORDS: HR MAS 1H NMR spectroscopy · Microalgae · Whole cell sample · Classification · Principal component analysis · Fuzzy clustering

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