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Marine Ecology Progress Series

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MEPS 275:115-128 (2004)  -  doi:10.3354/meps275115

Pigment specific in vivo light absorption of phytoplankton from estuarine, coastal and oceanic waters

P. A. Stæhr1,2,*, S. Markager1, K. Sand-Jensen2

1National Environmental Research Institute, Department of Marine Ecology, Frederiksborgvej 399, Box 358, 4000 Roskilde, Denmark
2Freshwater Biological Laboratory, University of Copenhagen, Helsingørgade 51, 3400 Hillerød, Denmark
*Present address: Hillerød. Email:

ABSTRACT: The influence of phytoplankton photoacclimation and adaptation to natural growth conditions on the chlorophyll a-specific in vivo absorption coefficient (a* ph) was evaluated for samples collected in estuarine, coastal and oceanic waters. Despite an overall gradient in the physio-chemical environment from estuaries, over coastal, to oceanic waters, no clear relationships were found between a* ph and the prevailing light, temperature, salinity and nutrient concentrations, indicating that short-term cellular acclimation was of minor importance for the observed variability in a* ph. The clear decline in a* ph from oceanic, over coastal, to estuarine waters was, however, strongly correlated with an increase in cell size and intracellular chlorophyll a (chl a) content of the phytoplankton, and a reduction of photosynthetic carotenoids relative to chl a. Variations in photoprotective carotenoids relative to chl a seemed to be of minor importance for the variability in a* ph. In addition, significant differences in phytoplankton composition and abundance were observed, primarily driven by an increase in the abundance of diatoms, which furthermore correlated with increasing pigment packaging and decreasing a* ph. The observed differences in a* ph were, therefore, primarily driven by longer-term adaptations of the phytoplankton community. Our data suggests that an overall increase in nutrient loading from oceanic to estuarine waters increases phytoplankton abundance and favors larger sized species, particularly within the diatoms. These changes eventually decrease a* ph through a rise in the package effect and a lower abundance of photosynthetic carotenoids relative to chl a.

KEY WORDS: Phytoplankton · Light absorption · Photoacclimation · Adaptation · Pigmentation · Community structure · Cell size

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