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Marine Ecology Progress Series

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MEPS 461:283-291 (2012)  -  DOI:

Stability of fatty acid composition in seal blubber during long-term storage

Ylva Lind1,*, Britt-Marie Bäcklin1, Karl Lundström2, Suzanne M. Budge3, Michael Walton4, Olle Karlsson

1Department of Contaminant Research, Swedish Museum of Natural History, PO Box 50007, 104 05 Stockholm, Sweden
2Department of Aquatic Resources, Swedish University of Agricultural Sciences, Turistgatan 5, 453 30 Lysekil, Sweden
3Canadian Institute of Fisheries Technology, Dalhousie University, Halifax, Nova Scotia B3J 2X4, Canada
4School of Biology, University of St Andrews, St Andrews KY16 8LB, UK

ABSTRACT: This study was performed to evaluate the stability of the fatty acid (FA) composition of blubber from Baltic grey seals Halichoerus grypus archived at −25°C in the Swedish Environmental Specimen Bank (SESB). Possible alterations of FA compositions with storage time and temperature were evaluated. Blubber from 5 seals was kept at +2°C and at −20°C and sampled for up to 6 mo and 33 mo, respectively. Furthermore, FA profile stability was evaluated in blubber stored in the SESB for 4 and 6 yr. This was done by re-sampling and re-analyzing blubber samples from SESB. In both parts of the study, a principal component analysis was performed on 48 FAs comprising 98 to 99% of the total FAs in blubber of Baltic grey seals. We found no indication of a critically altered FA composition, neither in blubber stored at +2°C for 6 mo nor in blubber stored at −20°C for 33 mo. The re-analysis of samples stored in SESB for ~4 to 6 yr only differed for a few FAs in some samples. The most remarkable differences were a slight increase in some long-chain unsaturated FAs (18:2n-6, 20:3n-3, 20:4n-3, 22:4n-3). As an increase in long-chain polyunsaturated FAs due to storage is highly unlikely, we believe that this is probably more due to the sampling technique than to a critically altered FA composition due to storage. Our results suggest that archived seal blubber may be safely used in retrospective analyses of diet using an FA signature analysis technique.

KEY WORDS: Fatty acid composition · Long-term storage · Blubber · Grey seal · Baltic Sea

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Cite this article as: Lind Y, Bäcklin BM, Lundström K, Budge SM, Walton M, Karlsson O (2012) Stability of fatty acid composition in seal blubber during long-term storage. Mar Ecol Prog Ser 461:283-291.

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