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Marine Ecology Progress Series

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MEPS 486:247-256 (2013)  -  DOI:

Effect of laser ablation depth in otolith life history scans

Reneé R. Hoover1,2,*, Cynthia M. Jones1

1Center for Quantitative Fisheries Ecology, Norfolk, Virginia 23508, USA
2Present address: Virginia Marine Resources Commission, Newport News, Virginia 23607, USA

ABSTRACT: Life history scans of fish otoliths are bringing new insight into the structure, connectivity, and movement of fish populations. Data obtained from such scans, however, possess inherent limitations that have not yet been fully addressed or understood. For example, several investigators have noted delays in otolith elemental uptake that do not appear to reflect habitat exposure. We hypothesized that the 3-dimensional structure of otoliths may produce sampling artifacts in the results obtained from laser ablation scans. To test this hypothesis, we sampled sagittal otoliths from juvenile Atlantic croaker Micropogonias undulatus with laser ablation inductively coupled plasma mass spectrometry to obtain elemental molar ratios of a common environmental marker (barium). We ablated 2 trenches of different depths on each otolith and performed spectral analyses on the data to investigate the effects of ablation depth, including differences in the periodicities and temporal variability between trenches. The mean barium concentration between the 2 trenches was significantly different (t = 114.25, p < 0.0001). From shallow to deep trenches, variance decreased; the standard error about the means was reduced from 0.609 to 0.086. Peaks in spectral density, which estimate the ingress timing for this species, were shifted in absolute value an average of 32 d. Our results highlight the necessity of considering depth of laser ablation when conducting life history scans.

KEY WORDS: Life history scan · LA-ICPMS · Lag effect · Otolith chemistry

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Cite this article as: Hoover RR, Jones CM (2013) Effect of laser ablation depth in otolith life history scans. Mar Ecol Prog Ser 486:247-256.

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