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Marine Ecology Progress Series

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MEPS 592:159-179 (2018)  -  DOI:

DNA sequencing of fish eggs and larvae reveals high species diversity and seasonal changes in spawning activity in the southeastern Gulf of California

Ana Luisa M. Ahern1,*, Jaime Gómez-Gutiérrez2, Octavio Aburto-Oropeza1, Ricardo J. Saldierna-Martínez2, Andrew F. Johnson1, Alice E. Harada1, Arturo R. Sánchez-Uvera2, Brad Erisman3, David I. Castro Arvizú4, Ronald S. Burton1

1Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, USA
2Departamento de Plancton y Ecología Marina, Centro Interdisciplinario de Ciencias Marinas, Instituto Politécnico Nacional, CP 23096, La Paz, Baja California Sur, Mexico
3Marine Science Institute, The University of Texas at Austin, Port Aransas, Texas 78373, USA
4Cabo Pulmo National Park, CP 23574, Los Cabos, Baja California Sur, Mexico
*Corresponding author:

ABSTRACT: Ichthyoplankton studies can provide valuable information on the species richness and spawning activity of fishes, complementing estimations done using trawls and diver surveys. Zooplankton samples were collected weekly between January and December 2014 in Cabo Pulmo National Park, Gulf of California, Mexico (n = 48). Ichthyoplankton is difficult to identify morphologically; therefore the DNA barcoding method was employed to identify 4388 specimens, resulting in 157 operational taxonomic units (OTUs) corresponding to species. Scarus sp., Halichoeres dispilus, Xyrichtys mundiceps, Euthynnus lineatus, Ammodytoides gilli, Synodus lacertinus, Etrumeus acuminatus, Chanos chanos, Haemulon flaviguttatum and Vinciguerria lucetia were the most abundant and frequent species recorded. Noteworthy species identified include rare mesopelagic species such as the giant oarfish Regalecus glesne and highly migratory and commercially important species such as black skipjack Euthynnus lineatus and yellowfin tuna Thunnus albacares. Spawning activities showed distinct seasonal patterns, with the highest abundance of ichthyoplankton recorded during spring, highest species richness during summer (90 OTUs) and lowest species richness during winter (28 OTUs). A total of 7 OTUs were recorded throughout the year (4.5%), 10 OTUs during 3 seasons (6.5%), 36 OTUs in 2 seasons (23%) and 104 OTUs were recorded in 1 season (66%). The study found eggs and/or larvae of 47 species that were not previously reported in Cabo Pulmo National Park. The results will allow resource managers to compare shifting populations and spawning patterns of species that may be affected by both conservation efforts and broader oceanographic changes associated with climate change.

KEY WORDS: Marine protected area · DNA barcoding · Molecular ecology · Marine conservation · Cabo Pulmo National Park · Gulf of California · Mexico

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Cite this article as: Ahern ALM, Gómez-Gutiérrez J, Aburto-Oropeza O, Saldierna-Martínez RJ and others (2018) DNA sequencing of fish eggs and larvae reveals high species diversity and seasonal changes in spawning activity in the southeastern Gulf of California. Mar Ecol Prog Ser 592:159-179.

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