Inter-Research > MEPS > v617-618 > p41-52  

MEPS 617-618:41-52 (2019)  -  DOI: https://doi.org/10.3354/meps12833

Molecular identification of the diet of Sardina pilchardus larvae in the SW Mediterranean Sea

Lidia Yebra1,*, Alma Hernández de Rojas2, Nerea Valcárcel-Pérez1, M. Carmen Castro2, Candela García-Gómez1, Dolores Cortés1, Jesús M. Mercado1, Raúl Laiz-Carrión1, Alberto García1, Francisco Gómez-Jakobsen1, Amaya Uriarte1, José M. Rodríguez2, José-María Quintanilla1

1Centro Oceanográfico de Málaga, Instituto Español de Oceanografía, 29640 Fuengirola, Spain
2Centro Oceanográfico de Gijón, Instituto Español de Oceanografía, 33212 Gijón, Spain
*Corresponding author:
Advance View was available online January 16, 2019

ABSTRACT: Molecular techniques provide new insights into the feeding strategies and diets of planktonic organisms such as the larvae of marine fish. We applied multiplex PCR to obtain the first estimates of the diets of larval European sardine Sardina pilchardus in the Alboran Sea (SW Mediterranean), where this species represents an important fishery resource. The feasibility of this technique was tested in a 26 h continuous survey of a shoal of larvae (10.80 ± 0.73 mm standard length, mean ± SD). Multiplex PCR was designed to detect the presence of 5 copepod species, a microplanktonic dinoflagellate (Gymnodinium) and the picoeukaryote algae family Prasinophyceae in larval guts. We simultaneously sampled sardine larvae and their potential prey (pico- to mesoplankton) and compared diel variability of the prey field and ingested items. Microplankton was dominated by flagellates, and copepods represented the most abundant mesozooplankton, reaching peak abundance at night. Prey DNA was detected throughout the entire diel cycle, despite no visible prey in the guts of larvae collected at night. Sardine larvae preyed on early life stages of the most abundant copepod species (Oncaea waldemari, Paracalanus indicus and Temora stylifera), suggesting an opportunistic foraging behaviour. The use of multiplex PCR allowed species-level identification of ingested nauplii and protists, which otherwise would remain unidentified.


KEY WORDS: Alboran Sea · Sardina pilchardus · Diel cycle · Larval ecology · Multiplex PCR


Full text in pdf format 
Cite this article as: Yebra L, Hernández de Rojas A, Valcárcel-Pérez N, Castro MC and others (2019) Molecular identification of the diet of Sardina pilchardus larvae in the SW Mediterranean Sea. Mar Ecol Prog Ser 617-618:41-52. https://doi.org/10.3354/meps12833

Export citation
Mail this link - Contents Mailing Lists - RSS
- -