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Marine Ecology Progress Series

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MEPS 628:95-104 (2019)  -  DOI: https://doi.org/10.3354/meps13084

Chemosynthetic bacterial signatures in Frenulata tubeworm Oligobrachia sp. in an active mud volcano of the Canadian Beaufort Sea

Dong-Hun Lee1, Jung-Hyun Kim2,*, Yung Mi Lee2, Young Keun Jin2, Charles Paull3, Dahae Kim2, Kyung-Hoon Shin1

1Hanyang University, 55 Hanyangdaehak-ro, Sangrok-gu, Ansan 15588, Republic of Korea
2Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea
3Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, California 95039, USA
*Corresponding author:

ABSTRACT: We performed bulk and compound-specific stable carbon isotope analyses to constrain specific carbon sources utilized for the chemosynthetic metabolisms of bacterial communities inhabiting the tube and worm of Oligobrachia sp. Together with bulk carbon isotopic compositions (-57.1 ± 1.2‰, mean ± SD) observed in the worm, the most depleted 13C values of predominant fatty acids (FAs) (i.e. C16:1ω7 [-71.4 ± 2.9‰] and C18:1ω7 [-76.7 ± 4.3‰]) indicated that sulfur-oxidizing symbionts were preferentially utilizing anaerobic oxidation of methane-derived dissolved inorganic carbon (-31.6 ± 4.2‰), rather than methane (-59.5 ± 3.9‰), as a carbon source. In contrast, the isotopic signatures of FAs of the tube sections indicated that both autotrophic and heterotrophic bacterial communities utilized dissolved inorganic carbon supplied from ambient bottom seawater and sediment porewater. In this regard, the metabolisms of chemosynthetic bacterial communities inhabiting the tube may be regarded as potentially supporting tubeworm nutrition. Given that the tubeworm host incorporates locally adapted microbial communities, the isotopic signatures suggest that different micro-niches identified from the tube and the worm, particularly in relation to complex metabolic interactions, may be correlated with in situ microbial processes in sediment and bottom seawater.


KEY WORDS: Mud volcano · Siboglinid tubeworm · Sherlock microbial identification system · MIDI · Fatty acids · Carbon isotopic composition · δ13C


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Cite this article as: Lee DH, Kim JH, Lee YM, Jin YK, Paull C, Kim D, Shin KH (2019) Chemosynthetic bacterial signatures in Frenulata tubeworm Oligobrachia sp. in an active mud volcano of the Canadian Beaufort Sea. Mar Ecol Prog Ser 628:95-104. https://doi.org/10.3354/meps13084

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