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Marine Ecology Progress Series

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MEPS 646:29-44 (2020)  -  DOI:

Seagrass leaf reddening alters the microbiome of Zostera muelleri

V. Hurtado-McCormick1,*, T. Kahlke1, D. Krix2, A. Larkum1, P. J. Ralph1, J. R. Seymour1

1University of Technology Sydney, Climate Change Cluster, Faculty of Science, Ultimo NSW 2007, Australia
2University of Technology Sydney, School of Life Sciences, Faculty of Science, Ultimo NSW 2007, Australia

ABSTRACT: Seagrasses host an extremely diverse microbiome that plays fundamental roles in seagrass health and productivity but may be sensitive to shifts in host physiology. Here, we observed a leaf reddening phenomenon in Zostera muelleri and characterized bacterial assemblages associated with green and reddened leaves to determine whether this change in leaf pigmentation stimulates shifts in the seagrass microbiome. Using 16S rRNA gene amplicon sequencing, we observed that the microbiome associated with 4 different leaf pigmentation categories (i.e. green, white, purple and black) differed significantly, with substantial changes in microbiome composition when the tissue is whitened (non-pigmented). Actinobacteria, Rhodobacteraceae, Erythrobacter, Sulfitobacter and Granulosicoccus were enriched in black and/or purple tissues and discriminated these microbiomes from those associated with green leaves. Contrastingly, all ‘discriminatory’ zero-radius operational taxonomic units (zOTUs) were depleted within the communities associated with white samples. While 40% of the abundant zOTUs identified were exclusively associated with a single pigmentation category, only 3% were shared across all categories, indicating partitioning of the phyllosphere microbiome. However, a significant proportion of the ‘normal’ (green) leaf core microbiome was also retained in the core communities associated with black (70%) and purple (70%) tissues. Contrastingly, no core zOTUs were maintained in the white tissues. These results indicate that environmentally driven physiological shifts in seagrasses, such as leaf reddening expressed in response to high irradiance, can impact the seagrass leaf microbiome, resulting in significant shifts in the microbiome of reddened leaves with the most extreme expression (in white tissue of reddened leaves).

KEY WORDS: Seagrass · Microbiome · 16S rRNA genes · Diversity-ecosystem functioning · Pigmentation · Irradiance

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Cite this article as: Hurtado-McCormick V, Kahlke T, Krix D, Larkum A, Ralph PJ, Seymour JR (2020) Seagrass leaf reddening alters the microbiome of Zostera muelleri. Mar Ecol Prog Ser 646:29-44.

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