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MEPS 649:175-188 (2020)  -  DOI: https://doi.org/10.3354/meps13451

Reconstruction of temperature experienced by Pacific bluefin tuna Thunnus orientalis larvae using SIMS and microvolume CF-IRMS otolith oxygen isotope analyses

Yulina Hane1,2,*, Shingo Kimura1,2, Yusuke Yokoyama2,3,4, Yosuke Miyairi2, Takayuki Ushikubo5, Toyoho Ishimura6,7, Nobuhiro Ogawa2, Tomoya Aono2,6, Kozue Nishida6,8

1Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277-8564, Japan
2Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277-8564, Japan
3Department of Earth and Planetary Science, Graduate School of Science, The University of Tokyo, Hongo, Tokyo 113-0033, Japan
4Organization for Programs on Environmental Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Tokyo 153-8902, Japan
5Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Nankoku, Kochi 783-8502, Japan
6National Institute of Technology, Ibaraki College, Hitachinaka, Ibaraki 312-8508, Japan
7Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan
8Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572, Japan
*Corresponding author:

ABSTRACT: This study aimed to reconstruct temperatures experienced during the larval period by adult Pacific bluefin tuna Thunnus orientalis using high-resolution otolith stable oxygen isotope (δ18O) analysis. A novel otolith sample preparation protocol for secondary ion mass spectrometry (SIMS) analysis developed in this study reduced the background noise of SIMS measurements, enabling analyses of >10 times higher resolution around the otolith core compared to previous studies using conventional isotope ratio mass spectrometry (IRMS). The values obtained from SIMS were compared to those obtained by microvolume δ18Ootolith analysis using micromilling and conventional continuous-flow IRMS (CF-IRMS). There was a systematic offset (average 0.41‰ with SIMS resulting in lower values) most likely caused by matrix effects on SIMS δ18Ootolith values that can be calibrated using a strong linear relationship between SIMS and CF-IRMS measurements (r2 = 0.78, p < 0.001). The core-to-edge δ18Ootolith of 5 Pacific bluefin tuna revealed fine-scale seasonal variations in water temperature agreeing with known migration patterns. In addition, the ambient water temperature experienced during larval stages (about 10-20 d post hatch) estimated from otolith core δ18O ranged from 26.7 to 30.7°C, overlapping with temperatures associated with the occurrence of larval Pacific bluefin tuna. Combining SIMS and microvolume CF-IRMS δ18O otolith analyses offers a microscale examination of fish ecology that is not possible with conventional IRMS techniques. This novel method is particularly useful for understanding the early life history of fish that may be affected by climate change and reconstructing a well-resolved migration history for fish species that have small otoliths and/or narrow growth increments.


KEY WORDS: Temperature reconstruction · Otolith · Oxygen isotope analysis · Secondary ion mass spectrometry · SIMS · continuous-flow isotope ratio mass spectrometry · CF-IRMS · Pacific bluefin tuna · Sample preparation protocol


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Cite this article as: Hane Y, Kimura S, Yokoyama Y, Miyairi Y and others (2020) Reconstruction of temperature experienced by Pacific bluefin tuna Thunnus orientalis larvae using SIMS and microvolume CF-IRMS otolith oxygen isotope analyses. Mar Ecol Prog Ser 649:175-188. https://doi.org/10.3354/meps13451

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