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Marine Ecology Progress Series

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MEPS 657:25-41 (2021)  -  DOI: https://doi.org/10.3354/meps13539

Characterization of diversity and community structure of small planktonic copepods in the Kuroshio region off Japan using a metabarcoding approach

Junya Hirai1,*, Keiichi Yamazaki2, Kiyotaka Hidaka3, Satoshi Nagai3, Yugo Shimizu3,4, Tadafumi Ichikawa3

1Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan
2Marine Fisheries Research and Development Center, Japan Fisheries Research and Education Agency, 6F Techno Wave 100, 1-1-25 Shin-urashima, Kanagawa, Yokohama, Kanagawa 220-0031, Japan
3Fisheries Resources Institute, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan
4Present address: Fishery Agency, 1-2-1 Kasumigaseki, Chiyoda, Tokyo 100-8907, Japan
*Corresponding author:

ABSTRACT: Small copepods are important prey for fish larvae in the Kuroshio region off southern Japan. However, revealing entire community structures of small copepods is difficult using conventional methods. We applied a metabarcoding method to size-fractionated community samples of epipelagic copepods (small: 0.1-0.5 mm, medium: 0.5-1.0 mm, and large: 1.0-2.0 mm). Samples were collected from 2013 to 2016 from the shoreward (Kuroshio Slope, KS) and oceanic (Kuroshio Gyre, KG) sides of the Kuroshio Current at 138° E; the results were compared with those in the center of the subtropical gyre (SG). The KS and KG sites showed both spatial differences and seasonal changes, with distinct differences between winter-spring and summer-autumn in each size-fractionated community. Water temperature markedly influenced copepod diversity and community structure, especially in the small size fraction. Warm-water species in the SG intruded into the Kuroshio regions during high-temperature periods, leading to high diversity in summer-autumn. Inter-annual environmental variations influenced by temperature and productivity were evident in KS, leading to clear changes in the sequence proportions of dominant small copepods including Paracalanus sp. and immature stages of the large copepod Calanus sinicus. Immature stages of medium/large copepods formed a substantial proportion of small-copepod communities in the Kuroshio regions (KS: 28.8%; KG: 24.7%; SG: 11.9%; based on average proportions of sequence reads). Because of their ecological importance and sensitivity to environmental changes, monitoring communities of small copepods with high taxonomic resolution may provide further insights into marine ecosystems, including fish recruitment, in the Kuroshio region.


KEY WORDS: Small copepods · Metabarcoding · Kuroshio · Diversity · Community structure


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Cite this article as: Hirai J, Yamazaki K, Hidaka K, Nagai S, Shimizu Y, Ichikawa T (2021) Characterization of diversity and community structure of small planktonic copepods in the Kuroshio region off Japan using a metabarcoding approach. Mar Ecol Prog Ser 657:25-41. https://doi.org/10.3354/meps13539

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