DAO 94:59-72 Supplementary Download

The parasite can be seen on a fragment of kidney, moving from one locations to another by attaching and releasing with its anterior prohaptor and posterior haptor.

Three parasites in well, prior to adding drugs. Their motion is typical of control parasites, relaxation, contraction and twisting around the longitudinal axis.

Normal activity of the anterior prohaptor.

Normal activity of the haptor.

The hooklets in the haptor move actively and separately.

CONT-6-egg-repro tract
Two parasites in tissue. One is moving, with an egg inside female reproductive system of the adult parasite.

The embryo, with prominent eye spots, is actively moving within the egg. The embryo has made rapidly moving cilia.

Ciliated hatchling (oncomiracidium) travels rapidly, occasionally spiraling up and down in the water column.

RX-1-reduced motion-less stretching
First video clip shows normal motion immediately after drug administration, second shows subsequent slowing and reduced relaxing and contracting.

RX-2-reduced motion-ends bend
This parasite continues to slowly move the haptor and prohaptor, but the mid-portion of body is immobile of slightly contracted.

RX-3-contracted-minor body stretch
This worm remains contracted, with occasional slight jerky elongation at the anterior end.

RX-4-contracted-minimal hooklet motion
These worms initially appear to be imobile, but the middle one is slowly and moving several hooklets.

RX-5-contracted-tremors 2 near dead
First video clip shows 3 parasites, right parasite is dead, top is barely moving, and lower parasite has slowed motion with intense tremors after contracting. Second video clip is a parasite with tremors.

RX-6-reduced motion-segmented
Segmented body appearance, with peristaltic type contractions along the mid portion of the body. This appearance wa s only rarely seen.